Cdk1 phosphorylates Drosophila Sas-4 to recruit Polo to daughter centrioles and convert them to centrosomes. Importantly, Sas-4 may have a more indirect role in mitotic PCM assembly in fly embryos as centriolar Sas-4 (as opposed to cytoplasmic Sas-4) is required to efficiently recruit Asl molecules around maturing mother centrioles, and Asl has an important role in recruiting Spd-2 and Cnn around mother centrioles Thus, PCM recruitment in fly embryos does not appear to require cytosolic S-CAP complexes. This study shows, however, that Cnn, Spd-2 and Asl are not recruited to the mother centriole as part of a complex with Sas-4. In a previous paper ( Conduit et al., 2014b) it was shown that one of these proteins, Cnn, and another key PCM-organising protein, Spd-2, are recruited around the mother centriole before spreading outwards to form a scaffold that supports mitotic PCM assembly the recruitment of Cnn and Spd-2 is dependent on another S-CAP protein, Asl. It has been reported that several important Drosophila PCM-organising proteins are recruited to centrioles from the cytosol as part of large cytoplasmic 'S-CAP' complexes that contain the centriole protein Sas-4. The mother centriole recruits and organises the PCM and templates the formation of the daughter centriole. PubMed ID: 26530814Ĭentrosomes have many important functions and comprise a 'mother' and 'daughter' centriole surrounded by pericentriolar material (PCM). Re-examining the role of Drosophila Sas-4 in centrosome assembly using two-colour-3D-SIM FRAP. Genetic map position - 3R: 2,977,076.2,979,886 Ĭlassification - T-complex protein 10 C-terminusĬonduit, P. Keywords - centriolar assembly, centriole replication, asymmetric cell division, flagellum biogenesis, cilium biogenesis, locomotion Gene name - Spindle assembly abnormal 4 ortholog (C.
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